Home | About Us | Current Issue | Ahead of print | Archives | Search | Instructions | Subscription | Feedback | Editorial Board | e-Alerts | Login 
Journal of Indian Association of Pediatric Surgeons
     Journal of Indian Association of Pediatric Surgeons
Official journal of the Indian Association of Pediatric Surgeons         
 Users Online:2630 
  Print this page Email this page   Small font sizeDefault font sizeIncrease font size

Table of Contents   
Year : 2019  |  Volume : 24  |  Issue : 3  |  Page : 192-196

Testicular volume in a cohort of prepubertal Indian children

1 Department of Paediatric Surgery, Christian Medical College, Vellore, Tamil Nadu, India
2 Department of Biostatistics, Christian Medical College, Vellore, Tamil Nadu, India

Date of Web Publication6-Jun-2019

Correspondence Address:
Dr. Rohith Srinivas
Department of Paediatric Surgery, 6th Floor, ISSCC Building, Christian Medical College, Ida Scudder Road, Vellore - 632 004, Tamil Nadu
Login to access the Email id

Source of Support: None, Conflict of Interest: None

DOI: 10.4103/jiaps.JIAPS_100_18

Rights and Permissions



Context: There are orchidometer-based testicular volume nomograms for Indian children; however, accurate and reliable values measured by ultrasound are lacking.
Aims: The aim of this study was to (1) measure the testicular volumes of boys from birth to 8 years and generate reference values and (2) to identify factors if any that may influence variation in testicular volumes.
Settings and Design: This was a prospective observational study conducted on 320 children in the Department of Pediatric Surgery, Christian Medical College, Vellore, India.
Subjects and Methods: A total of 320 boys without any genital abnormalities were studied. The testes were scanned using a linear transducer, and the length, width, and depth of each testis were recorded. Testicular volume was calculated using Lambert's equation – length × width × depth × 0.71.
Statistical Analysis Used: Mean testicular volumes and standard deviation for every year of age were calculated. The centile values for testicular volume were computed using R software.
Results: Age-specific nomogram of each testis was created separately. Interobserver variability of the measurement was shown to be up to 0.3 ml. No difference was demonstrated in the testicular volumes between the right and left testis. No correlation was found between body weight and body mass index with testicular volume. From the data on differences in size between the two sides, a volume differential index of 27% corresponds to the 95th centile.
Conclusions: Reference values have been created for testicular volumes in prepubertal Indian children that could be used to assess the effects of disease and surgical interventions in this age group.

Keywords: Nomogram, orchidometer, testis, ultrasound

How to cite this article:
Srinivas R, Thomas RJ, Sebastian T, Kurian JJ. Testicular volume in a cohort of prepubertal Indian children. J Indian Assoc Pediatr Surg 2019;24:192-6

How to cite this URL:
Srinivas R, Thomas RJ, Sebastian T, Kurian JJ. Testicular volume in a cohort of prepubertal Indian children. J Indian Assoc Pediatr Surg [serial online] 2019 [cited 2022 Aug 8];24:192-6. Available from: https://www.jiaps.com/text.asp?2019/24/3/192/259745

   Introduction Top

Accurate determination of testicular volume is an important tool in the clinical assessment and follow-up of conditions such as disorders of puberty, undescended testis, and varicocele.[1],[2] Ultrasonographic measurement of testicular volume is now considered the gold standard as they are highly accurate and precise.[3],[4],[5],[6]

Various reported studies in Indian children have used the Prader's orchidometer[7] and water-displacement technique[8] to measure testicular volume, however, none have reference values for ultrasonography which is the gold standard. This study aims to obtain ultrasonographic reference values of testicular volumes for Indian children from birth to 8 years of age.

   Subjects and Methods Top

This prospective observational study was conducted on 320 children from March 2015 to February 2016. All inpatient and outpatient children from birth to 8 years of age were considered for the study. Children with a history of groin surgery, inguinal hernia, hydrocele, undescended testis, varicocele, testicular tumors, torsion testes, disorders of sexual differentiation, chronic liver disease, and failure to thrive were excluded from the study. The minimum sample size required for <4 years of age was 35 each per year of age, and for 4–8 years was 45 each per year of age. The reason attributed to larger sample size at higher ages was increasing variance.

Children were stratified into different age groups according to their date of birth. Children aged 1 day–365 days were grouped as age one, 366–730 days were grouped as age two, 731 days–1095 days as age three, etc. A detailed explanation of the nature of the study was provided to the caregivers, and an informed consent in their own language was obtained. The study design and methodology were approved by the Ethics Committee and the Institutional Review Board (Ref-IRB Min No 9342 dated March 03, 2015).

Sonographic assessment of testicular volume was performed only by the principal investigator (PI), thereby negating the interobserver bias. The instrument used was Sonosite M-turbo portable ultrasound machine with the L25x/13-6 MHz, 25-mm linear array transducer. The procedure was performed with adequate privacy, in supine position with the thighs apart. Distractions such as toys and colorful objects aided in the process of performing the scan. Scanning was performed using light pressure to avoid distorting the testicular shape. Separate axial and longitudinal images of each testis were obtained, and the largest dimension was taken and used for calculation of testicular volume. Testicular volumes were calculated using Lambert's formula (length [L] × width [W] × height [H] × 0.71) as it is considered to be the most accurate and precise.[9],[10]

On analysis, in modeling, log-normal distribution was assumed to get over any skewness. Cubic spline smoothing was done using the generalized additive models for location scale and shape method which in turn uses the R software (University of Auckland, New Zealand).[11] The 5th, 10th, 25th, 50th, 75th, 90th, and 95th centiles for testicular volume, mean, and standard deviations (SDs) were computed using R software. These were then represented on graphs. In addition, testicular volume was compared with weight and body mass index (BMI) using the paired t-test, and the significance of these correlations was calculated using the two-tailed significance test (P < 0.05 taken to be statistically significant).

To validate the ultrasound findings of the PI, ten boys were chosen at random and ultrasonographic measurement of the testicular volume was done separately by a consultant radiologist and the PI. The findings of the PI and the radiologist were then compared to calculate the level of agreement between them.

   Results Top

The volume of the right and left testis for each individual was measured. The right testis volume ranged from 0.49 ml to 2.07 ml, and the left testis volume ranged from 0.47 ml to 2.04 ml. There was no statistically significant difference between the volumes of the right and left testis for all age groups. Individual and combined nomograms of testicular volumes ranging between the 5th and 95th centile were made [Figure 1], [Figure 2], [Figure 3]. The centile values, mean, and SDs for the right and left testicular volumes along with the combined average are given below [Table 1], [Table 2], [Table 3].
Figure 1: Testicular volume distribution in the right testis across various age groups

Click here to view
Figure 2: Testicular volume distribution in the left testis across various age groups

Click here to view
Figure 3: Combined testicular volume nomogram from birth to 8 years

Click here to view
Table 1: Centile, mean, and standard deviation values according to the age for the right testis

Click here to view
Table 2: Centile, mean, and standard deviation values according to the age for the left testis

Click here to view
Table 3: Combined centile, mean, and standard deviation values according to the age for both testis

Click here to view

In addition, the presence of any correlation between testicular volume and variables, such as weight of the child and BMI, was studied. It was found that there was no statistically significant correlation between the weight or BMI of boys and testicular volume.

When comparing the measurements made by the PI and the radiologist, a minimum and maximum volume difference of 0.07 ml and 0.38 ml, respectively, was calculated. This difference in measurements between the PI and radiologist was not statistically significant as the inter-rater agreement was excellent and the values were within ± 2SD.

Although no statistically significant difference was found between the testicular volumes of the right and left side in our study, we sought to use the difference in volumes between the sides to create an index that could be used to identify children with potential testicular diseases and to assess postoperative testicular outcomes. The volume differential index (VDI) was thus calculated as the difference in volumes between the two testes/volume of the larger testis × 100. It was expressed as a percentage and then plotted on a graph [Figure 4]. Mean and SDs for VDI were calculated, and from the graph, it was extrapolated that ± 2SD from the mean would be 27%. Thus, a VDI of more than 27% may be considered as a pointer to abnormal testicular function needing further evaluation.
Figure 4: Mean difference in testicular volume and volume differential index

Click here to view

   Discussion Top

Testicular volume measurement is an important tool in diagnosing a variety of medical and surgical conditions. Men with infertility usually undergo a thorough assessment of testicular volume along with semen analysis, as it correlates well with the functioning of the testis.[12],[13] Studies have shown that men with infertility who record a subnormal sperm density usually have a combined/total testicular volume of <30 ml whereas men who had azoospermia and severe oligospermia had combined/total testicular volumes <10 ml and 20 ml, respectively.[12]

Volume measurements also aid in making important clinical decisions regarding the surgical intervention. In boys with varicocele, a difference in volume of 20%–25% between the two testes has been used as a marker for surgical correction. As semen analysis in the younger age group is considered unethical and psychologically incorrect, the measurement of testicular volume can help in deciding the need for surgery.[4] Testicular volume measurement has also been useful in the postoperative monitoring and long-term follow-up of the patients after surgery for varicocele and undescended testis.[14]

A precise determination of testicular volume is of paramount importance in evaluating patients who suffer from various disease conditions that affect the growth and function of the testis. Although these nomograms exist for children of the Western population, reliable nomograms for Indian or Asian children are not available. The few Indian studies done in the past for testicular volume nomograms have used the Prader's orchidometer which gives a less than accurate measure, and the age of children recruited were always 8 years and above. Hence, obtaining reliable nomograms of testicular volume for Indian children <8 years of age has become imperative.

The study by Kuijper et al.[15] created nomograms for boys across various ethnic groups from birth to 6 years of age laying special emphasis on infants. Testicular volumes in this study ranged from 0.23 to 0.5 ml. Similarly, in the study conducted by Goede et al.[16] on children between 6 months and 18 years of age, testicular volume for the right testis ranged from 0.23 to 20.23 ml and the left testis from 0.2 to 20.13 ml.

However, in our study, testicular volumes from birth to 8 years of age were higher than other studies.[15],[16] This can be attributed to the different formulae used in the calculation of testicular volume. Whereas the above-mentioned studies[15],[16] used the ellipsoid formula in calculating the testicular volume, we used Lambert's formula in this study which has been proven as the most accurate of all formulas after comparison with the water-displacement technique.[9],[10] This technique is based on the Archimedes' principle and is considered to be the gold standard. Here, the testis is bared by excising the cord structures and epididymis. The testis is then immersed in a beaker of water, and the volume of water displaced is recorded as the testicular volume.[17] The requirement of an orchidectomy specimen for this technique makes it unavailable for everyday use.

In addition, an interobserver variation of up to 0.3 ml was recognized between the PI and radiologist which was within ± 2SD. This variation in testicular size can be attributed to technical differences where even the slightest of pressure can distort the shape of the testis and thus its dimensions.[18] However, the volumes obtained in our study at 8 years of age (95th centile volume of 1.4812 ml [L] − 1.4919 ml [R]) were found to be comparable with other Indian studies[7],[8] where the testicular volume at 8 years of age was 1.4 ml.

In our study, we also tried to demonstrate whether there existed any correlation between testicular volume and body weight or BMI. As suggested by previous studies,[15],[19] no correlation was found. Our study also demonstrated that there is no significant difference in the volumes between the right and left testis (P > 0.05) in concordance with studies from the Western world.[15],[16] Hence, a combined nomogram of both testes should suffice for a particular age group.

The difference in volume between the two testes was used to create an index that could be used to identify children with potential testicular diseases and use to assess postoperative testicular outcomes. The VDI was thus calculated as the difference in volumes between the two testes/volume of the larger testis × 100 and was expressed as a percentage. The VDI for all boys in our study was calculated and plotted on a graph. The 95th centile or two SDs from the mean were found to be 27%.

The index has many potential benefits. It could be used as a quick guide to identify “normal” boys with no apparent testicular pathology but with an abnormal testicular size. Boys with varicocele on regular follow-up could be offered surgery if the VDI exceeds 27%. In children with undescended testis and varicocele, testicular atrophy can occur either due to the disease process itself or as a consequence of surgery. VDI done both in the preoperative and postoperative periods will help in prognosticating these children.

   Conclusions Top

Measurement of the testicular volume is a useful tool to assess testicular function. Baseline values for testicular volume have been lacking in Indian children. We have constructed a nomogram of testicular volumes with centiles and SD applicable to Indian children up to 8 years of age.

Testicular VDI of 27% was found to be equivalent to the 95th centile in our study. The index could be the basis for surgery in children with varicocele and also be helpful in assessing postoperative outcomes in varicocele and undescended testis.

Financial support and sponsorship


Conflicts of interest

There are no conflicts of interest.

   References Top

Osifo OD, Evbuomwan I. Undescended testes in a developing country: A study of the management of 71 patients. Afr J Urol 2008;5:11-4.  Back to cited text no. 1
Zucchi A, Mearini L, Mearini E, Fioretti F, Bini V, Porena M, et al. Varicocele and fertility: Relationship between testicular volume and seminal parameters before and after treatment. J Androl 2006;27:548-51.  Back to cited text no. 2
Behre HM, Nashan D, Nieschlag E. Objective measurement of testicular volume by ultrasonography: Evaluation of the technique and comparison with orchidometer estimates. Int J Androl 1989;12:395-403.  Back to cited text no. 3
Costabile RA, Skoog S, Radowich M. Testicular volume assessment in the adolescent with a varicocele. J Urol 1992;147:1348-50.  Back to cited text no. 4
Rivkees SA, Hall DA, Boepple PA, Crawford JD. Accuracy and reproducibility of clinical measures of testicular volume. J Pediatr 1987;110:914-7.  Back to cited text no. 5
Diamond DA, Paltiel HJ, DiCanzio J, Zurakowski D, Bauer SB, Atala A, et al. Comparative assessment of pediatric testicular volume: Orchidometer versus ultrasound. J Urol 2000;164:1111-4.  Back to cited text no. 6
Lall KB, Singhi S, Gurnani M, Chowdhary B, Garg OP. Normal testicular volume in school children. Indian J Pediatr 1980;47:389-93.  Back to cited text no. 7
Jit I, Sanjeev. The volume of the testes in North-West Indian children. Hum Biol 1988;60:945-51.  Back to cited text no. 8
Sakamoto H, Saito K, Oohta M, Inoue K, Ogawa Y, Yoshida H, et al. Testicular volume measurement: Comparison of ultrasonography, orchidometry, and water displacement. Urology 2007;69:152-7.  Back to cited text no. 9
Mbaeri TU, Orakwe JC, Nwofor AM, Oranusi CK, Mbonu OO. Ultrasound measurements of testicular volume: Comparing the three common formulas with the true testicular volume determined by water displacement. Afr J Urol 2013;19:69-73.  Back to cited text no. 10
Stasinopoulos DM, Rigby RA. Generalized additive models for location scale and shape (GAMLSS) in R. J Stat Softw 2007;23:1-46.  Back to cited text no. 11
Arai T, Kitahara S, Horiuchi S, Sumi S, Yoshida K. Relationship of testicular volume to semen profiles and serum hormone concentrations in infertile Japanese males. Int J Fertil Womens Med 1998;43:40-7.  Back to cited text no. 12
Takihara H, Cosentino MJ, Sakatoku J, Cockett AT. Significance of testicular size measurement in andrology: II. Correlation of testicular size with testicular function. J Urol 1987;137:416-9.  Back to cited text no. 13
David OO, Iyekoretin E. Undescended testes in a developing country: A study of the management of 71 patients. Afr J Paediatr Surg 2008;5:11-4.  Back to cited text no. 14
[PUBMED]  [Full text]  
Kuijper EA, van Kooten J, Verbeke JI, van Rooijen M, Lambalk CB. Ultrasonographically measured testicular volumes in 0- to 6-year-old boys. Hum Reprod 2008;23:792-6.  Back to cited text no. 15
Goede J, Hack WW, Sijstermans K, van der Voort-Doedens LM, Van der Ploeg T, Meij-de Vries A, et al. Normative values for testicular volume measured by ultrasonography in a normal population from infancy to adolescence. Horm Res Paediatr 2011;76:56-64.  Back to cited text no. 16
Bhat S, Sathyanarayana Prasad M, Giridhar A, Srinivasa Y, Paul F. Testicular volume measurement: comparison of prader's orchidometry, ultrasonography, and actual volume by water displacement. J Integr Nephrol Androl 2016;3:92-5.  Back to cited text no. 17
  [Full text]  
Taskinen S, Taavitsainen M, Wikström S. Measurement of testicular volume: Comparison of 3 different methods. J Urol 1996;155:930-3.  Back to cited text no. 18
Handelsman DJ, Staraj S. Testicular size: The effects of aging, malnutrition, and illness. J Androl 1985;6:144-51.  Back to cited text no. 19


  [Figure 1], [Figure 2], [Figure 3], [Figure 4]

  [Table 1], [Table 2], [Table 3]

This article has been cited by
1 Ultrasonographic measurements of the testicular volume in Turkish boys aged 0–8 years and comparison with international references
Edis Çolak, Behzat Özkan
Journal of Pediatric Endocrinology and Metabolism. 2022; 0(0)
[Pubmed] | [DOI]
2 Referential Values of Testicular Volume Measured by Ultrasonography in Normal Children and Adolescents: Z-Score Establishment
Chen Liu, Xiao Liu, Xiangxiang Zhang, Boyang Yang, Lan Huang, Hongying Wang, Hongkui Yu
Frontiers in Pediatrics. 2021; 9
[Pubmed] | [DOI]
3 Testicular Volume in Children with Sickle Cell Anaemia in Uyo, a South-South Nigerian City
Obasi Onwuka Okorie, Michael Eteng Eyong, Udeme Ekpenyong Ekrikpo, Emmanuel Eyo Ekanem
Journal of Tropical Pediatrics. 2021; 67(4)
[Pubmed] | [DOI]
4 Testicular volume in a cohort of prepubertal Indian children
MahmoodDhahir Al-Mendalawi
Journal of Indian Association of Pediatric Surgeons. 2020; 25(2): 126
[Pubmed] | [DOI]


Print this article  Email this article


    Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
    Article in PDF (834 KB)
    Citation Manager
    Access Statistics
    Reader Comments
    Email Alert *
    Add to My List *
* Registration required (free)  

   Subjects and Methods
    Article Figures
    Article Tables

 Article Access Statistics
    PDF Downloaded114    
    Comments [Add]    
    Cited by others 4    

Recommend this journal

Contact us | Sitemap | Advertise | What's New | Copyright and Disclaimer | Privacy Notice

 İ 2005 - Journal of Indian Association of Pediatric Surgeons | Published by Wolters Kluwer - Medknow 

Online since 1st May '05